Covalent cross-linking of the Phytophthora megasperma oligopeptide elicitor to its receptor in parsley membranes.
Identifieur interne : 002B87 ( Main/Exploration ); précédent : 002B86; suivant : 002B88Covalent cross-linking of the Phytophthora megasperma oligopeptide elicitor to its receptor in parsley membranes.
Auteurs : T. Nürnberger [Allemagne] ; D. Nennstiel ; K. Hahlbrock ; D. ScheelSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1995.
Descripteurs français
- KwdFr :
- Cellules cultivées (MeSH), Cinétique (MeSH), Daucus carota (métabolisme), Données de séquences moléculaires (MeSH), Extraits de plantes (biosynthèse), Fixation compétitive (MeSH), Fragments peptidiques (composition chimique), Fragments peptidiques (métabolisme), Fragments peptidiques (pharmacologie), Glycoprotéines (MeSH), Glycoprotéines membranaires (isolement et purification), Glycoprotéines membranaires (métabolisme), Magnoliopsida (métabolisme), Membranes intracellulaires (métabolisme), Microsomes (métabolisme), Phytophthora (métabolisme), Protéines fongiques (isolement et purification), Protéines fongiques (métabolisme), Protéines membranaires (MeSH), Radio-isotopes de l'iode (MeSH), Réactifs réticulants (pharmacologie), Sesquiterpènes (MeSH), Soja (métabolisme), Spécificité du substrat (MeSH), Succinimides (pharmacologie), Séquence d'acides aminés (MeSH), Terpènes (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
- biosynthèse : Extraits de plantes.
- composition chimique : Fragments peptidiques.
- isolement et purification : Glycoprotéines membranaires, Protéines fongiques.
- métabolisme : Daucus carota, Fragments peptidiques, Glycoprotéines membranaires, Magnoliopsida, Membranes intracellulaires, Microsomes, Phytophthora, Protéines fongiques, Soja.
- pharmacologie : Fragments peptidiques, Réactifs réticulants, Succinimides.
- Cellules cultivées, Cinétique, Données de séquences moléculaires, Fixation compétitive, Glycoprotéines, Protéines membranaires, Radio-isotopes de l'iode, Sesquiterpènes, Spécificité du substrat, Séquence d'acides aminés, Terpènes, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Binding, Competitive (MeSH), Cells, Cultured (MeSH), Cross-Linking Reagents (pharmacology), Daucus carota (metabolism), Electrophoresis, Polyacrylamide Gel (MeSH), Fungal Proteins (isolation & purification), Fungal Proteins (metabolism), Glycoproteins (MeSH), Intracellular Membranes (metabolism), Iodine Radioisotopes (MeSH), Kinetics (MeSH), Magnoliopsida (metabolism), Membrane Glycoproteins (isolation & purification), Membrane Glycoproteins (metabolism), Membrane Proteins (MeSH), Microsomes (metabolism), Molecular Sequence Data (MeSH), Peptide Fragments (chemistry), Peptide Fragments (metabolism), Peptide Fragments (pharmacology), Phytophthora (metabolism), Plant Extracts (biosynthesis), Sesquiterpenes (MeSH), Soybeans (metabolism), Substrate Specificity (MeSH), Succinimides (pharmacology), Terpenes (MeSH).
- MESH :
- chemical , biosynthesis : Plant Extracts.
- chemical , chemistry : Peptide Fragments.
- chemical , isolation & purification : Fungal Proteins, Membrane Glycoproteins.
- chemical , metabolism : Fungal Proteins, Membrane Glycoproteins, Peptide Fragments.
- chemical , pharmacology : Cross-Linking Reagents, Peptide Fragments, Succinimides.
- metabolism : Daucus carota, Intracellular Membranes, Magnoliopsida, Microsomes, Phytophthora, Soybeans.
- Amino Acid Sequence, Binding, Competitive, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Glycoproteins, Iodine Radioisotopes, Kinetics, Membrane Proteins, Molecular Sequence Data, Sesquiterpenes, Substrate Specificity, Terpenes.
Abstract
An oligopeptide elicitor from Phytophthora megasperma f.sp. glycinea (Pep-13) that induces phytoalexin accumulation in cultured parsley cells was radioiodinated and chemically cross-linked to its binding site in microsomal and plasma membrane preparations with each of three homobifunctional reagents. Analysis by SDS/PAGE and autoradiography of solubilized membrane proteins demonstrated labeling of a 91-kDa protein, regardless of which reagent was used. Cross-linking of this protein was prevented by addition of excess unlabeled Pep-13. The competitor concentration found to half-maximally reduce the intensity of the cross-linked band was 6 nM, which is in good agreement with the IC50 value of 4.7 nM, obtained from ligand binding assays. No crosslinking of 125I-labeled Pep-13 was observed by using microsomal membranes from three other plant species, indicating species-specific occurrence of the binding site. Coupling of 125I-Pep-13 to the parsley 91-kDa protein required the same structural elements within the ligand as was recently reported for binding of 125I-Pep-13 to parsley microsomes, elicitor-induced stimulation of ion fluxes across the plasma membrane, the oxidative burst, the expression of defense-related genes, and phytoalexin production. These findings suggest that the 91-kDa protein identified in parsley membranes is the oligopeptide elicitor receptor mediating activation of a multicomponent defense response.
DOI: 10.1073/pnas.92.6.2338
PubMed: 7892267
PubMed Central: PMC42478
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(metabolism)</term><term>Membrane Proteins (MeSH)</term><term>Microsomes (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Peptide Fragments (chemistry)</term><term>Peptide Fragments (metabolism)</term><term>Peptide Fragments (pharmacology)</term><term>Phytophthora (metabolism)</term><term>Plant Extracts (biosynthesis)</term><term>Sesquiterpenes (MeSH)</term><term>Soybeans (metabolism)</term><term>Substrate Specificity (MeSH)</term><term>Succinimides (pharmacology)</term><term>Terpenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules cultivées (MeSH)</term><term>Cinétique (MeSH)</term><term>Daucus carota (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Extraits de plantes (biosynthèse)</term><term>Fixation compétitive (MeSH)</term><term>Fragments peptidiques (composition chimique)</term><term>Fragments peptidiques (métabolisme)</term><term>Fragments peptidiques (pharmacologie)</term><term>Glycoprotéines (MeSH)</term><term>Glycoprotéines membranaires (isolement et purification)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Magnoliopsida (métabolisme)</term><term>Membranes intracellulaires (métabolisme)</term><term>Microsomes (métabolisme)</term><term>Phytophthora (métabolisme)</term><term>Protéines fongiques (isolement et purification)</term><term>Protéines fongiques (métabolisme)</term><term>Protéines membranaires (MeSH)</term><term>Radio-isotopes de l'iode (MeSH)</term><term>Réactifs réticulants (pharmacologie)</term><term>Sesquiterpènes (MeSH)</term><term>Soja (métabolisme)</term><term>Spécificité du substrat (MeSH)</term><term>Succinimides (pharmacologie)</term><term>Séquence d'acides aminés (MeSH)</term><term>Terpènes (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" 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Proteins</term><term>Molecular Sequence Data</term><term>Sesquiterpenes</term><term>Substrate Specificity</term><term>Terpenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Fixation compétitive</term><term>Glycoprotéines</term><term>Protéines membranaires</term><term>Radio-isotopes de l'iode</term><term>Sesquiterpènes</term><term>Spécificité du substrat</term><term>Séquence d'acides aminés</term><term>Terpènes</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An oligopeptide elicitor from Phytophthora megasperma f.sp. glycinea (Pep-13) that induces phytoalexin accumulation in cultured parsley cells was radioiodinated and chemically cross-linked to its binding site in microsomal and plasma membrane preparations with each of three homobifunctional reagents. Analysis by SDS/PAGE and autoradiography of solubilized membrane proteins demonstrated labeling of a 91-kDa protein, regardless of which reagent was used. Cross-linking of this protein was prevented by addition of excess unlabeled Pep-13. The competitor concentration found to half-maximally reduce the intensity of the cross-linked band was 6 nM, which is in good agreement with the IC50 value of 4.7 nM, obtained from ligand binding assays. No crosslinking of 125I-labeled Pep-13 was observed by using microsomal membranes from three other plant species, indicating species-specific occurrence of the binding site. Coupling of 125I-Pep-13 to the parsley 91-kDa protein required the same structural elements within the ligand as was recently reported for binding of 125I-Pep-13 to parsley microsomes, elicitor-induced stimulation of ion fluxes across the plasma membrane, the oxidative burst, the expression of defense-related genes, and phytoalexin production. These findings suggest that the 91-kDa protein identified in parsley membranes is the oligopeptide elicitor receptor mediating activation of a multicomponent defense response.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7892267</PMID><DateCompleted><Year>1995</Year><Month>04</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0027-8424</ISSN><JournalIssue CitedMedium="Print"><Volume>92</Volume><Issue>6</Issue><PubDate><Year>1995</Year><Month>Mar</Month><Day>14</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>Covalent cross-linking of the Phytophthora megasperma oligopeptide elicitor to its receptor in parsley membranes.</ArticleTitle><Pagination><MedlinePgn>2338-42</MedlinePgn></Pagination><Abstract><AbstractText>An oligopeptide elicitor from Phytophthora megasperma f.sp. glycinea (Pep-13) that induces phytoalexin accumulation in cultured parsley cells was radioiodinated and chemically cross-linked to its binding site in microsomal and plasma membrane preparations with each of three homobifunctional reagents. Analysis by SDS/PAGE and autoradiography of solubilized membrane proteins demonstrated labeling of a 91-kDa protein, regardless of which reagent was used. Cross-linking of this protein was prevented by addition of excess unlabeled Pep-13. The competitor concentration found to half-maximally reduce the intensity of the cross-linked band was 6 nM, which is in good agreement with the IC50 value of 4.7 nM, obtained from ligand binding assays. No crosslinking of 125I-labeled Pep-13 was observed by using microsomal membranes from three other plant species, indicating species-specific occurrence of the binding site. Coupling of 125I-Pep-13 to the parsley 91-kDa protein required the same structural elements within the ligand as was recently reported for binding of 125I-Pep-13 to parsley microsomes, elicitor-induced stimulation of ion fluxes across the plasma membrane, the oxidative burst, the expression of defense-related genes, and phytoalexin production. These findings suggest that the 91-kDa protein identified in parsley membranes is the oligopeptide elicitor receptor mediating activation of a multicomponent defense response.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nürnberger</LastName><ForeName>T</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nennstiel</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Hahlbrock</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Scheel</LastName><ForeName>D</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003432">Cross-Linking Reagents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006023">Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007457">Iodine Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008565">Membrane Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012717">Sesquiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013388">Succinimides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013729">Terpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C091510">elicitor protein, Phytophthora megasperma</NameOfSubstance></Chemical><Chemical><RegistryNumber>37297-20-4</RegistryNumber><NameOfSubstance UI="C011991">phytoalexins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EVY5D0U6SH</RegistryNumber><NameOfSubstance UI="C011240">dithiobis(succinimidylpropionate)</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001667" MajorTopicYN="N">Binding, Competitive</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003432" MajorTopicYN="N">Cross-Linking Reagents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018552" MajorTopicYN="N">Daucus carota</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006023" MajorTopicYN="N">Glycoproteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007425" MajorTopicYN="N">Intracellular Membranes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007457" MajorTopicYN="N">Iodine Radioisotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019684" MajorTopicYN="N">Magnoliopsida</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008565" MajorTopicYN="N">Membrane Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008861" MajorTopicYN="N">Microsomes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012717" MajorTopicYN="N">Sesquiterpenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013388" MajorTopicYN="N">Succinimides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013729" MajorTopicYN="N">Terpenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>3</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>3</Month><Day>14</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>3</Month><Day>14</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">7892267</ArticleId><ArticleId IdType="pmc">PMC42478</ArticleId><ArticleId IdType="doi">10.1073/pnas.92.6.2338</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Biol Chem. 1993 Jul 15;268(20):14724-31</Citation><ArticleIdList><ArticleId IdType="pubmed">8325850</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1992 Jul 10;70(1):21-30</Citation><ArticleIdList><ArticleId IdType="pubmed">1623521</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1984 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Hahlbrock</name><name sortKey="Nennstiel, D" sort="Nennstiel, D" uniqKey="Nennstiel D" first="D" last="Nennstiel">D. Nennstiel</name><name sortKey="Scheel, D" sort="Scheel, D" uniqKey="Scheel D" first="D" last="Scheel">D. Scheel</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Nurnberger, T" sort="Nurnberger, T" uniqKey="Nurnberger T" first="T" last="Nürnberger">T. Nürnberger</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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